Nickel Chelate on Glass

Nickel chelate slides and cover slips have NTA (nitrilotriacetic acid) in the nickel form covalently attached to the surface.   This surface is specific for binding his-tagged molecules, particularly those with a 6-his tail. The bond is quite strong but can be disrupted by high concentrations of imidazole.  The binding takes place preferably in neutral or slightly basic solution and the bond is stable over a wide pH range.

We have available from stock standard clear microscope slides 25 x 75 mm,1 mm thick and glass cover slips 22 x 40mm, number 1 1/12.   We can also add nickel NTA on any other size and configuration that is commercially available as a custom product.   We have created this surface on cover glass ranging in size from 12 mm circles to 24 x 60 mm rectangles.   We have also reacted small glass chips and beads.Please call or email with your specific needs.

The products are coated on both sides, so they can be used either way.

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